DBPapers
DOI: 10.5593/sgem2017/42/S17.003

ACTIVE SLUDGE AND STRAINS ISOLATED FROM IT AS BIOAGENTS IN BIOFUEL CELLS

D.I. Stom, E. Yu. Konovalova, G.O. Zhdanova, M.Yu. Tolstoy, O.F. Vyatchina
Tuesday 12 September 2017 by Libadmin2017

References: 17th International Multidisciplinary Scientific GeoConference SGEM 2017, www.sgem.org, SGEM2017 Conference Proceedings, ISBN 978-619-7408-07-2 / ISSN 1314-2704, 29 June - 5 July, 2017, Vol. 17, Issue 42, 19-26 pp, DOI: 10.5593/sgem2017/42/S17.003

ABSTRACT

The technology of microbial fuel cells (MFC) makes it possible to obtain electricity by eliminating and utilizing wastewater components [7]. The promising biological agents in the MFC are microbial associations. The presence of strains of different species of microorganisms allows consortia to consume a much wider range of compounds. Multicomponent makes them more resistant to negative environmental influences. An example of such associations is the sludge of treatment facilities. As part of most wastewater entering the treatment facilities, there are surfactants, which can have a negative impact on organisms of the sludge. The aim of the work was to study the ability of the sludge, as well as the strains Micrococcus luteus and Serratia marcescens isolated from it, to generate electricity in the MFC when utilizing model wastewater. Also studied the effect of surfactant - sodium dodecyl sulfate (SDS) on the viability and activity of electrogenic studied bioagents [2]. The study was carried out in the MFC constructions developed by us. These constructions allowed access to the MFC content without affecting its sterility and anaerobic properties. In the experiments, the voltage, the current in the MFC were measured and the power was calculated. Along with this, the charge of electrodes, pH and Ox-Red medium, the number of viable cells was fixed. Experiments have shown that the bioagents under study have a high electrogenic activity. In this case, the voltage generated by the sludge, M. luteus and S. marcescens during 24 hours, was 540±81.0, 500±75.0 and 455±68.2 mV, respectively. The inhibition of test-functions of bioagents occurred already in the presence of 0.01% surfactant. A complete inhibition of the ability to generate current and growth cells of sludge and M. luteus was observed at 0.1% SDS, S. marcescens at 0.05%. Thus, the M. luteus and S. marcescens isolated from the sludge can be used as bioagents in wastewater treatment and generation of electricity in MFC, provided that the concentration of SDS in the effluents does not exceed for M. luteus 0.1%, for S. marcescens 0.05%.

Keywords: microbial fuel cells, Serratia marcescens, Micrococcus luteus, active sludge, surfactant.