DBPapers
DOI: 10.5593/sgem2017/61/S25.094

HPLC-DAD CHARACTERIZATION AND CAROTENOID CONTENT OF SEA BUCKTHORN JUICE

G. Stanciu, I.A. Oancea, S. Lupsor, A. Popescu
Tuesday 12 September 2017 by Libadmin2017

References: 17th International Multidisciplinary Scientific GeoConference SGEM 2017, www.sgem.org, SGEM2017 Conference Proceedings, ISBN 978-619-7408-12-6 / ISSN 1314-2704, 29 June - 5 July, 2017, Vol. 17, Issue 61, 719-728 pp, DOI: 10.5593/sgem2017/61/S25.094

ABSTRACT

It’s well known that sea buckthorn berries are rich in vitamins, carotenoids, flavonoids, and other biologically active components with significant therapeutically properties.The main polyphenolic compounds from sea buckthorn fresh juice were identified and quantified using HPLC-DAD method and the β-carotene content was determined using the spectrophotometric method.The extractions were performed using four different solvents. The sea buckthorn juice was fractional extracted using: diethyl ether (Fraction A), methanol (Fraction B), HCl 10% (Fraction C) and hot water (Fraction D).The separation, identification and quantification of the phenolic compounds were done using an adapted USP30 HPLC method.The content of β-carotene was determined in Fraction A using the spectrophotometric method performed at 450 nm.The results indicate the presence of β-carotene in Fraction A (7.012 mg/100 mL fresh juice) and also the presence of valuable phenolic compounds found in all four studied fractions of sea buckthorn fresh juice.

Keywords: sea buckthorn, HPLC-DAD, β-carotene, Hippophae rhamnoides