DBPapers
DOI: 10.5593/SGEM2016/HB63/S08.033

EVALUATION OF ANALYTICAL PROPERTIES OF THE DIAGNOSTIC KITS FOR DETECTION OF VIRUS-SPECIFIC ANTIBODIES FROM THE IGG CLASS IN BOVINE BLOOD SERUM BY THE ENZYME IMMUNOASSAY

A. K. Barsukov, A. I. Kuznetsov, O. Y. Nesterova, K. K. Sharafullin
Friday 11 November 2016

References: 16th International Multidisciplinary Scientific GeoConference SGEM 2016, SGEM Vienna GREEN Extended Scientific Sessions, www.sgemviennagreen.org, SGEM2016 Conference Proceedings, ISBN 978-619-7105-79-7 / ISSN 1314-2704, 2 - 5 November, 2016, Book 6 Vol. 3, 255-260pp, DOI: 10.5593/SGEM2016/HB63/S08.033

ABSTRACT
A spectrum of different companies’ diagnostic kits designed on the basis of indirect enzyme immunoassay schemes and aimed at the detection of antibodies to the same infectious agent was studied. The best serial samples can be characterized by the amount of the adsorption immobilized agent and the activity of antibodies in the anti-specific immune peroxidase conjugate. We studied immune enzyme kits designed to detect antibodies specific to the leukemia virus or the infectious bovine rhinotracheitis virus in cattle blood serum. For this purpose, according to the laboratory scheme an electrophoretically homogeneous and chromatographically monomeric IgG form was isolated from bovine blood plasma. The bovine IgG samples were subjected to standard immobilization based on adsorption on polystyrene plates. Immune peroxidase conjugates from the kits designed to detect antibodies to the same viral agent led to a standard dilution in a direct method of the ELISA. Standard dilution means conjugate dilution ensuring the formation of optical density in the range (1.1 ± 0.1) with a suitable substrate. Standard dilutions of immune peroxidase conjugates were incubated in the substrate excess to register the kinetics of enzymatic reaction products forming. The amount of the adsorption-immobilized viral antigen was assessed by optical density using crossover incubating of the basic immune reagents from different producers, but all of them were designed for the detection of antibodies to the same virus infection.
We consider it is possible to use the outlined technology to form the optimal combination of screening tests with the kits having the best analytical properties to enhance infectious safety of pharmaceutical plasma biologics.

Keywords: diagnostic kits, quality evaluation of the basic immune reagents.

PAPER DOI: 10.5593/SGEM2016/HB63/S08.033, EVALUATION OF ANALYTICAL PROPERTIES OF THE DIAGNOSTIC KITS FOR DETECTION OF VIRUS-SPECIFIC ANTIBODIES FROM THE IGG CLASS IN BOVINE BLOOD SERUM BY THE ENZYME IMMUNOASSAY

35 EURO ADD TO CART


Your cart ( items)

Subtotal:
Tax cost ():
Shipping cost:
Total:
Checkout Empty