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DOI: 10.5593/SGEM2016/HB63/S08.026

BIOCHEMICAL FUNDAMENTAL PRINCIPLE FOR IMPROVING PRODUCTION TECHNOLOGIES AIMED AT IMMUNOGLOBULIN BIOLOGICAL PRODUCTS AGAINST INFECTIOUS DISEASES OF ANIMALS

A. K. Barsukov,A. I. Kuznetsov, O. Y. Nesterova, K. K. Sharafullin
Friday 11 November 2016

References: 16th International Multidisciplinary Scientific GeoConference SGEM 2016, SGEM Vienna GREEN Extended Scientific Sessions, www.sgemviennagreen.org, SGEM2016 Conference Proceedings, ISBN 978-619-7105-79-7 / ISSN 1314-2704, 2 - 5 November, 2016, Book 6 Vol. 3, 201-206pp, DOI: 10.5593/SGEM2016/HB63/S08.026

ABSTRACT
The antigenic uniqueness of bovine IgG has been evaluated. Bovine immune serum is used in pharmaceutical bioindustry as a therapeutic and preventive agent for prevention and treatment of infectious diseases in various animal species. Electrophoretically homogeneous and chromatographically monomeric IgG samples were prepared from the blanks of specific plasma or blood serum. We prepared rabbit immune serum specific for bovine IgG, which was used in the double immune diffusion reaction and for the synthesis of anti-species immune peroxidase conjugate. Enzyme immunoassay was performed basing on the direct method and the direct competitive methods with the usage of anti-species immune peroxidase conjugate or homologous IgGs labeled with horseradish peroxidase. Immunological identity of specific IgG was evaluated by constructing a calibration relation in a homologous enzyme immunoassay. The calibration relation of the direct ELISA method was represented by the calibration samples from 1 ng/ml up to the maximum possible concentration of 10 μg/ml of IgG. In competitive analysis the calibration relation was represented by native samples of 10 ng/ml up to 1 mg/ml. To our mind the comparative analysis of the ELISA results obtained within the limits of the calibration relation for the homologous system of indication in various configurations allows to discuss the presence of identical and structurally similar epitopes in the composition of specific IgGs. According to the results of double immune diffusion and the direct method of the enzyme immunoassay the highest level of antigenic homology is found between the IgG donor (cows) and the yak. In competitive analyses this trend remains unchanged, but the level of homology between the donor IgG and the recipient IgG is significantly reduced. Taking into account the achievements of fundamental virology and the decryption of mechanisms of viral reassortant forming we believe it is necessary to transfer therapeutic and prophylactic immune serums into the category of raw materials for the development of virus inactivating stages and associated technologies of directed immunoglobulin biologics, which are represented as electrophoretically homogeneous and chromatographically monomeric forms of target proteins with the highest possible level of homology between the IgG donor and the recipient.

Keywords: human and animal IgG, enzyme immunoassay

PAPER DOI: 10.5593/SGEM2016/HB63/S08.026, BIOCHEMICAL FUNDAMENTAL PRINCIPLE FOR IMPROVING PRODUCTION TECHNOLOGIES AIMED AT IMMUNOGLOBULIN BIOLOGICAL PRODUCTS AGAINST INFECTIOUS DISEASES OF ANIMALS

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