DBPapers
DOI: 10.5593/SGEM2016/HB63/S08.025

BACTERIAL COMMUNITY COMPOSITION CHANGES IN THE PROCESS OF OILY WASTE REMEDIATION USING

P. Galitskaya, L. Biktasheva, S. Selivanovskaya
Friday 11 November 2016

References: 16th International Multidisciplinary Scientific GeoConference SGEM 2016, SGEM Vienna GREEN Extended Scientific Sessions, www.sgemviennagreen.org, SGEM2016 Conference Proceedings, ISBN 978-619-7105-79-7 / ISSN 1314-2704, 2 - 5 November, 2016, Book 6 Vol. 3, 193-200pp, DOI: 10.5593/SGEM2016/HB63/S08.025

ABSTRACT
Oily wastes are considered to be one of the most serious environmental problems. Development of an effective and environmentally friendly strategy of oily wastes remediation still remains a challenge. Usage of composts from organic wastes for oily wastes remediation may be one of the effective strategies desired. In this study, we estimated the changes of bacterial community composition in the process of bioremediation of the oily sludge containing 125 g kg-1 total petroleum hydrocarbons by means of compost prepared from municipal organic wastes (organic fraction of municipal solid waste, wastewater sludge and sawdust polluted by oil, 1:3:1). Oily sludge and compost were mixed in a ratio 1:2. During 120 days of remediation in lab conditions, total petroleum hydrocarbons content decreased from 40 to 19 g kg-1. Since bacteria are reported to be a driven force of petroleum decomposition, we estimated the structure of bacterial community in the beginning (1st day), middle (42nd day) and end (120th day) of bioremediation. As revealed by Illumina MiSeq method, Protebacteria (mainly, Delta- and Alpha) was a dominating phylum in all the samples. In the all samples except oily sludge, Bacteroides and Actinobacteria were subdominants, while in the compost Bacteroides and Firmicutes were subdominats. As follows from NMDS analysis, bacterial community structure in the 1st-day mixture was similar to the compost’s one but not to the oily waste’s one. Sludge microorganisms such as Porphyromonadaceae, Alteromonadaceae, Flavobacteriaceae and Clostridiales in the bioremediation mixtures disappeared because are most of them anaerobic. Bacterial community structure after 42 days of incubation was close but not similar to those in the compost and in the 1st-day mixture, and significantly differed from them after 120 days.

Keywords: bioremediation, bacterial community, oily waste

PAPER DOI: 10.5593/SGEM2016/HB63/S08.025, BACTERIAL COMMUNITY COMPOSITION CHANGES IN THE PROCESS OF OILY WASTE REMEDIATION USING COMPOST

35 EURO ADD TO CART


Your cart ( items)

Subtotal:
Tax cost ():
Shipping cost:
Total:
Checkout Empty