DBPapers
DOI: 10.5593/SGEM2016/B61/S25.094

RESEARCH ON SPERM QUALITY OF NORTH AMERICAN STURGEON SPECIES POLYODON SPATHULA

M.Costache
Wednesday 7 September 2016 by Libadmin2016

References: 16th International Multidisciplinary Scientific GeoConference SGEM 2016, www.sgem.org, SGEM2016 Conference Proceedings, ISBN 978-619-7105-68-1 / ISSN 1314-2704, June 28 - July 6, 2016, Book6 Vol. 1, 715-722 pp

ABSTRACT

The paper presents research on sperm quality and affecting factors in male of North American sturgeon species Polyodon spathula recently acclimated in Romania. Quality assessment was performed by microscopic and macroscopic analysis of sperm from a total of 5 males used in artificial spawning experiments in 2015. Hormonal stimulation of breeders was accomplish by receiving Nerestin 5A synthetic hormone in a single dose of 0,1 mg/kg of body weight. Microscopic analysis was to determine the concentration of sperm by haemocytometer method, sperm shaped and size determination using electronic microscopy and identification of specific staining, motility determination using dark field microscopy, testing the viability of sperm after sperm activation. Sperm quality parameters were interpreted by correlation with following factors: hormonal stimulation, the spawning season, temperature, extenders properties, short or long term storage of sperm, the biology of spawning (age, weight, length), health and wellness condition. Total sperm per male ranged between 4.5 and 49.4 x 109. Although sperm volume is high even at four, five days after stimulation, sperm count decreases. In what concerns motility, it was found that the percentage of motile spermatozoa was higher after activating sperm with Saline solution until water activation technology.

Keywords: Polyodon spathula, spawning, sperm quality