DBPapers
DOI: 10.5593/SGEM2016/B61/S25.089

OPTIMIZATION PROTOCOL FOR THE EXTRACTION OF ANTIMICROBIAL COMPONENTS FROM VEXIBIA ALOPECUROIDES ROOTS

T.Kustova, T.Karpenyuk, Y.Tufuminova, A.Goncharova
Wednesday 7 September 2016 by Libadmin2016

References: 16th International Multidisciplinary Scientific GeoConference SGEM 2016, www.sgem.org, SGEM2016 Conference Proceedings, ISBN 978-619-7105-68-1 / ISSN 1314-2704, June 28 - July 6, 2016, Book6 Vol. 1, 677-684 pp

ABSTRACT

Quantity and activity of plant extracts are dependent on extraction protocol. Many factors such as solvent, temperature, number of extraction steps, liquid-to-solid ratio and the particle size contribute to the efficacy of the extraction process. The objective of this study was to optimize the conditions (solvent: methylene chloride, ethanol; extraction time: from 12 to 36 hours; liquid-to-solid ratio: 4:1, 8:1, 10:1 ml/g; and particle size: 3, 5, 7 mm) for the extraction of antimicrobial compounds from Vexibia alopecuroides roots and to measure total flavonoid contents and antimicrobial activity of obtained extracts. Results of this study shown the best extraction protocol: the solvent was methylene chloride; best extraction time were found 24 hours, the liquid-to-solid ratio were found 10:1 ml/g, and the particle size - 7 mm which rendered a total flavonoid content of 13.5 μg of QE/mg of extract with the highest antibacterial activity against Staphylococcus aureus (IC50 3.05 μg/ml) and Methicillin-resistant S. aureus (IC50 2.9 μg/ml). This study can be useful in the development of pharmaceutical extraction processes, including further studies concerning the optimal number of sequential steps to enhance the efficacy of a large-scale extraction system.

Keywords: Extract, antimicrobial activity, roots