Sunday 1 August 2010 by Libadmin2010

10th International Multidisciplinary Scientific GeoConference - SGEM2010, www.sgem.org, SGEM2010 Conference Proceedings/ ISBN 10: 954-91818-1-2, June 20-26, 2010, Vol. 2, 669-676 pp


Alternative toxicological tests (microbiotests) are those that reduce the number of
animals in a toxicity test, refine procedures to make them less painful or stressful,
replace animals with non-animal systems, or replace one animal species with another
that is lower on the phylogenetic scale. A number of useful alternative methods have
been developed and accepted for the evaluation of the potential toxic effects of
chemicals products and environment samples. The literature analysis concerning the use
of microbiotests for the control of chemicals products and environmental samples, show
that these are in full development because of environmental pollution monitoring and
control increasingly complexity requirements and in the same time to comply the
necessity of new experimental methodology with low cost and reduced complexity, but
with relevant, reproducible and qualitative results.

To be conform to the new requirements impose by the European Regulation REACH
concerning chemicals registrationv, assessment, authorization and restriction, we have
analyzed the possibility to reduce / replace the biotests which use fish with
microbiotests which use bacteria (Vibrio fischeri sp.), crustacean (Daphnia magna sp.)
and algae (Selenastrum capricornutum sp.). This paper presents some comparative
testing studies performed for environmental sample and chemicals using both
conventional toxicity tests with fish and alternative tests - microbiotest. The results
obtained have lead to conclusion that the use of microbiotests are very practical and
offer a good reproducibility and relevant results, but also is not recommended to
renounce definitively to the conventional toxicity tests with vertebrate organisms (ex.
fish) because these represent the principal target of pollution. The toxicity tests with
vertebrate have an important role in risk assessment, where can be use in combination
with microbiotests. The use of the multi - species microbiotest battery, which can offer
relevant answers concerning the chemicals / chemical products / environmental samples
risk, may lead to an economical and readily assessment of acute and chronic toxicity.

Keywords: chemicals, toxicity, microbiotest, REACH