DBPapers
DOI: 10.5593/SGEM2014/B61/S25.075

THE KNOX GENES INVOLVEMENT IN THE DEVELOPMENT OF MULTILEAFLED TRAIT ON TETRAPLOID MEDICAGO SATIVA

Popescu S., Boldura O.M., Botau D.
Wednesday 1 October 2014 by Libadmin2014

References: 14th International Multidisciplinary Scientific GeoConference SGEM 2014, www.sgem.org, SGEM2014 Conference Proceedings, ISBN 978-619-7105-20-9 / ISSN 1314-2704, June 19-25, 2014, Book 6, Vol. 1, 551-558 pp

ABSTRACT
The deciphering of the metabolic pathway which induce the multileaflet trait in alfalfa it is of great importance due to the economic importance of the high biomass and the increased protein content. For this purpose the knox genes involved in meristem development and leaf morphogenesis were investigated. Thus, the knox genes expression were comparative analyzed for trileaflets plant and multileaflet ones in order to determine their specific involvement in leaf development using the semi quantitative one-step RT-PCR method. Four types of tissues were investigated: shoot tips, young leaves, old leaves and roots to determine the knox genes function in different stages of tissues development. It turned out that the average of the knox genes expression for all of the experimental variants was significantly higher for the mulileaflet plants, compared with the trileaflet ones.

Keywords: alfalfa, knox genes, RT-PCR