DBPapers
DOI: 10.5593/SGEM2014/B61/S25.071

THE CROSS-POLLINATION STUDY BETWEEN GENETICALLY MODIFIED AND CONVENTIONAL CORN CROPS IN ROMANIA

Bonea D., Urechean V., Soare R., Bonciu E.
Wednesday 1 October 2014 by Libadmin2014

References: 14th International Multidisciplinary Scientific GeoConference SGEM 2014, www.sgem.org, SGEM2014 Conference Proceedings, ISBN 978-619-7105-20-9 / ISSN 1314-2704, June 19-25, 2014, Book 6, Vol. 1, 519-526 pp

ABSTRACT
In Romania, the GM commercial crops are limited to MON 810 corn that is resistant to the European corn borer (ECB, Ostrinia nubilalis). This GM corn is authorized for
cultivation since 2007 (when Romania became a member of the European Union). To ensure the coexistence, the adventitious presence of the genetically modified organisms must not exceed the level of 0.9 % established by Regulation (EC) 1829/2003. Because the corn is a species with female and male flowers formed in separate parts of the same plant, the gene flow between plants is high. The pollen – mediated gene flow of corn can be monitored by measurement the rate of cross-pollination. The objective of this study was the cross-pollination rate between GM and conventional corn by xenia effect. For this, three experimental fields were established at ARDS Simnic. Within the first experience, where the MON 810 event was planted at the centre of the experimental field and surrounded by conventional corn as receiver, the rate of cross-pollination ranged from 0.83% (plot N-E) to 3.72% (plot W). Within the second experience, where the receiver corn was planted at the centre of the experimental field and surrounded by the donor MON 810, there was recorded the highest rate of cross - pollination, respectively of 27.62%. In a third experiment where they used different isolation distances, cross-pollination rates were between 1.40 and 1.74% for 10 – 19.8 m distance, between 0.77 and 0.94% for 20 – 29.8 m distance and between 0.90 and 0.94% for 30-30.8 m distance. The high levels of cross - pollination rate has been determined by: the compatibility gametophyte at genotypes studied, the coincidence in flowering donor and receiver corn, the isolation distance as well as the prevailing wind direction of pollination period. Under these circumstances, the coexistence of the genetically modified and conventional corn it is not possible, excepting the situation when we use isolation distances for over 40 m or other strategies.

Keywords: MON 810 event; gene flow; isolation distance; xenia effect.