DBPapers
DOI: 10.5593/SGEM2014/B61/S25.070

STUDY OF VARIABILITY INDUCED BY IN VITRO CULTURES AT BLUEBERRY POPULATIONS

Botau D., Bolda V., Popescu S., Boldura O.M.
Wednesday 1 October 2014 by Libadmin2014

References: 14th International Multidisciplinary Scientific GeoConference SGEM 2014, www.sgem.org, SGEM2014 Conference Proceedings, ISBN 978-619-7105-20-9 / ISSN 1314-2704, June 19-25, 2014, Book 6, Vol. 1, 511-518 pp

ABSTRACT
In vitro cells culture is a good method to induce somatic variability in plants. The
selection of plant tissues with a good capacity of cellular biosynthesis is necessary to obtain the important secondary metabolites by in vitro culture system. In our experiments we used biochemical methods to evaluate the variability induced by in vitro callus culture at three blueberry populations native in Romania. Callus obtained from Arieseni, Retezat and Valea Sebeşului populations, on WPM medium was sub-cultured in presence of ANA, BAP and AS. Antioxidant capacity using the FRAP method, glutathion (GSH), total anthocyanin, macroelements, microelements and heavy metals contents from callus lines selected were investigated. The AS concentration positively affects the activity of glutathione, with a significantly positive correlation coefficient (r = 0.54, p <0.05). The use of this hormone facilitates the obtaining of blueberry callus biomass with an increased antioxidant activity. There was also found a strong positive correlation between the total content of anthocyanin and FRAP values (r = 0.60, p <0.05) and a positive correlation with the level of GSH, respectively (r = 0.47, p <0.05).

Keywords: callus, native blueberry populations, biochemical methods