DBPapers
DOI: 10.5593/SGEM2014/B61/S25.046

CORRELATION BETWEEN IN VITRO CYTOTOXICITY AND ANTIOXIDANT ACTIVITY OF SOME NEW BIOADHESIVE GELS BASED ON CHLORHEXIDINE METALLIC COMPLEXES

Negreanu – Pirjol T., Roncea F., Negreanu-Pirjol BS..,Sanda J., Sirbu R., Moldovan L., Miresan H.
Wednesday 1 October 2014 by Libadmin2014

References: 14th International Multidisciplinary Scientific GeoConference SGEM 2014, www.sgem.org, SGEM2014 Conference Proceedings, ISBN 978-619-7105-20-9 / ISSN 1314-2704, June 19-25, 2014, Book 6, Vol. 1, 329-336 pp

ABSTRACT
The new bioadhesive gels obtained in this paper aim to diversify the market of
semisolid dosage forms with oropharyngeal mucosa action, covers a general need of human and veterinary health area for their disinfectant and antioxidant properties and could be used for oral hygiene. Were prepared several innovative semisolid preparations type bioadhesive gels, based on synthesized Cu (II) and Zn complex compounds of chlorhexidine salts incorpored in gelatin, glycerol and sorbitol as excipients. The obtained bioadhesive gels were evaluated regarding their physical-chemical properties from compendial sources, such as: appearance, homogenity, pH, spread-ability and penetration capacity, using standard methods according to European Pharmacopoeia. In vitro cytotoxicity of these bioadhesive gels on fibroblast cultures were determined. In this order were analyzed the cellular viability by MTT method and cellular morphology. At concentrations in range of 100 μg/mL – 1 mg/mL we noticed no effect in morphology fibroblast modification and a 95% viability after 24 h. The antioxidant activity of these bioadhesive gels comparative to Cu (II) and Zn complex compounds of chlorhexidine was determined by chemiluminescence method, based on the multiple acceleration of a natural reaction leading to the generation of a superoxide anion radical. The antioxidant capacity was quantified by comparison with standard luminol, using a Turner Design TD 20/20 SUA apparatus, at λ 420 nm wavelength. From the obtained results we noticed that comparative to the chlorhexidine complexes with copper (II) and zinc, the new bioadhesive gels had an increased antioxidative capacity (84-89%), which suggest them as efficient antioxidant agents, with low toxicity on fibroblasts.

Keywords: bioadhesive gel, chlorhexidine, Cu(II) and Zn complexes, cytotoxicity, antioxidant activity