DBPapers
DOI: 10.5593/SGEM2014/B51/S20.054

ISOLATION AND IDENTIFICATION OF BACTERIA ISOLATES FROM ARSENIC CONTAMINATED ANTHROPOSOILS

K. Petkova, H. Vojtkova, L. Jurkovic, P. Ferianc, M. Remenar
Wednesday 1 October 2014 by Libadmin2014

References: 14th International Multidisciplinary Scientific GeoConference SGEM 2014, www.sgem.org, SGEM2014 Conference Proceedings, ISBN 978-619-7105-17-9 / ISSN 1314-2704, June 19-25, 2014, Book 5, Vol. 1, 399-404 pp

ABSTRACT
This study aims to isolate and identify autochthonous bacteria from arseniccontaminated soils collected in the locality of Zemianske Kostoľany. After the coal ash pond spill in 1965, soils, sediments, and ground water were all significantly contaminated with arsenic exceeding 1500 ppm and with other potentially toxic elements. Firstly, sixty-three morphologically different bacterial isolates were obtained from samples of contaminated substrates. Consequently, identification of 16S rRNA sequences showed identities to genus Pseudomonas, Rhodococcus, Bacillus, Streptomyces and Chryseobacterium. Out of these, Pseudomonas was the genus of the greatest proportion of isolates in the studied ashy-soil substrates (62.5 %). The genus was represented by the species Pseudomonas sp., P. chlororaphis, P. putida, P. baetica and P. reinekei. The results showed that the genus Bacillus had the second highest abundance (20.83 %) and was represented by species B. cereus and B. pumilus. Two isolates of the genus Bacillus were classified as uncultured soil bacterium clone. There were further recorded two isolates of the genus Chryseobacterium. The genus Streptomyces and Rhodococcus were represented by only one species. Last but not least, 20 % of isolates were not identified to their genus. Results of studied bacterial species diversity, which was able to adapt to living in contaminated environment, are the basis for the application of selected indigenous bacterial species in bioleaching process as one of the potential methods for bioremediation of arsenic-contaminated soils.

Keywords: arsenic, bacterial species diversity, bioremediation, contamination