DBPapers
DOI: 10.5593/SGEM2014/B51/S20.014

BIOCHEMICAL CHANGES INDUCED BY LEAD CONTAMINATION OF BIOLOGICAL SAMPLES MULBERRY (MORUS SP.)

C. Diaconescu, L. Vidu, L. Urdes, D. Popa, M. Marin, D. Stefan
Wednesday 1 October 2014 by Libadmin2014

References: 14th International Multidisciplinary Scientific GeoConference SGEM 2014, www.sgem.org, SGEM2014 Conference Proceedings, ISBN 978-619-7105-17-9 / ISSN 1314-2704, June 19-25, 2014, Book 5, Vol. 1, 97-104 pp

ABSTRACT
Research conducted on pollution / contamination of soils with heavy metals revealed several remedial options including cultivation of high capacity to absorb pollutants such as mulberry (Morus sp.) Heavy metals can affect the germination and development of mulberry plants (Morus sp.) and can induce biochemical changes on lipids and antioxidant enzymes. Therefore this paper aims: -highlighting the impact of lead contamination processes mulberry plant germination and development. Seeds of mulberry were germinated in an aqueous medium with the addition of 0.4 mg Pb /100 ml solution of Pb (NO3) 2 and 0, 04 mg Pb / 100ml solution compared to the control. Were used mulberry seedlings pricking out of which the growth substrate has been contaminated with lead the same concentrations as in the case of seed. - investigate the lipid peroxidation level and antioxidant enzymes activity superoxide dismutase-SOD, glutathione peroxidase -GPX and catalase-CAT influenced by the accumulation of lead in plant mulberry leaf biomass. Experimental results have shown that the ex-situ conditions, Pb accumulation of contaminants influence the germination of seeds in terms of its blocking at concentrations greater than 0.4 mg% ml Pb solution. Concentration range from 0.0 to 0.04 mg Pb% ml did not influence germination and mulberry plants has no phenotypic changes. The degree of lipid peroxidation was significantly increased and the activity of antioxidant enzymes SOD, CAT and GPX decreased significantly (P <0.05) in version with the addition of 0.4 mg Pb / 100ml solution, compared to treatment with the addition of 0.04 mg Pb/100 ml and the control group. Lead concentrations greater than 0.4 mg Pb/100ml lead nitrate solution causes blocking the germination of seedlings visible phenotypic changes with poor prognosis and significant biochemical changes.

Keywords: lead contamination, Morus sp., lipid peroxidation, superoxide dismutase