DBPapers
DOI: 10.5593/SGEM2014/B32/S13.024

INFLUENCE OF SAPROPEL (GYTTJA) ON TRIFOLIUM PRATENSE SEEDS GERMINATION IN PRESENCE OF COPPER

K. Stankevica, Z. Vincevica-Gaile, M.Klavins
Wednesday 1 October 2014 by Libadmin2014

References: 14th International Multidisciplinary Scientific GeoConference SGEM 2014, www.sgem.org, SGEM2014 Conference Proceedings, ISBN 978-619-7105-14-8 / ISSN 1314-2704, June 19-25, 2014, Book 3, Vol. 2, 175-182 pp

ABSTRACT
The terms “sapropel” or “gyttja” are used to describe bottom deposits of freshwater bodies rich in the organic matter (organic matter content exceed 15%) which are formed from the remains of water animals and vegetation mixed with mineral components. Sapropel has a fine disperse and plastic structure and it can be used in various fields such as agriculture, livestock farming, balneology, cosmetology etc. Germination tests are used for determination of germination potential and quality of a selected seed lot. Several factors such as diseases, age of seeds, mechanical damage of seeds, excessive moisture or drought during storage can affect the behaviour of seeds germination. The rate of germination is very significant as it indicates field planting value of seeds. Detection of influencing factors and their intensity on seed germination is especially significant research due to its practical importance in agriculture and gardening. The aim of this study was to determine the influence of sapropel (gyttja) on the germination of Trifolium pratense (red clover) seeds in the presence of copper contamination. Methodology of the study involved selection and preparation of Trifolium pratense seeds, selection of peaty sapropel obtained from Lake Pilvelis (Latvia) and preparation of germination tests. To assess the influence of sapropel on seeds germination half of seeds were germinated in water, while another part – in sapropel. In addition, to estimate the interaction of sapropel with metallic elements the study was done by adding copper in various Cu2+ target concentration (i.e., 0, 40, 70, 100, 130, 200, 400, 600, 800, 1000 mg/L) into water or sapropel. Seed germination was done by using “Phytotestkit” plates. Seeds were germinated in thermostat at the temperature 22°C for 7 days. Developed radicles, hypocotyls and shoots were measured. Obtained results showed positive influence of sapropel on Trifolium pratense seeds germination and development as well as sapropel addition reduced negative effects caused by metal contamination on seeds endurance affirming potential use of sapropel as effective soil amendment.

Keywords: copper, germination, gyttja, sapropel, Trifolium pratense

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