DOI: 10.5593/SGEM2014/B32/S13.012


I. Charousova, S.Javorekova, J.Medo, J. Makova, S. Kovacsova
Wednesday 1 October 2014 by Libadmin2014

References: 14th International Multidisciplinary Scientific GeoConference SGEM 2014, www.sgem.org, SGEM2014 Conference Proceedings, ISBN 978-619-7105-14-8 / ISSN 1314-2704, June 19-25, 2014, Book 3, Vol. 2, 83-90 pp

Three different organic substrates (compost, bottom sediment and biosludge) were applied to the arable soil in the laboratory conditions (incubation time 105 days at 25°C) in doses of 20 t.ha-1 and 80 t.ha-1. The main objective of the present study was to observe the changes in numbers of bacterial and fungal colonies using classic plate dilution method, changes in genetic microbial biodiversity using Polymerase Chain Reaction - Denaturing Gradient Gel Electrophoresis (PCR-DGGE) and changes in the microbial enzymatic activity (dehydrogenase, FDA - hydrolase and phosphatase activity) at the beginning (1st day) and at the end (105th day) of the experiment. The statistically significant differences in amount of bacteria (ANOVA, Tukey test, P0.05) were mostly demonstrated on the 1st day of the experiment. All three tested substrate inputs rapidly increased percentage representation of the monitored bacteria. In case of microscopic fungi, the most significant changes were determined after the addition of bottom sediment, which in both used doses increased number of fungal colonies, particularly the number representation of microscopic fungus Penicillium simplicissimum at the end of the experiment. According to the results of the cluster analysis of PCR-DGGE, the diversity of bacteria from enriched soil was at least influenced by the addition 20 as well as 80 t.ha-1 of compost (similarity from 48 to 62 %). On the other hand, the addition of biosludge (both doses) influenced bacterial diversity at the most (similarity 33 %). In case of microscopic fungi, all three tested substrates affected fungal diversity approximately equally (similarity from 30 to 50 %). According to the results of regression analysis, P˂ 0.05, high relative abundance of bacteria as well as fungi determined by classic plate dilution method positively correlated with microbial diversity determined by PCR-DGGE and also with dehydrogenase activity.

Keywords: biofertilizers, microbial community, PCR-DGGE, enzymatic activity

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