DBPapers
DOI: 10.5593/sgem2011/s20.118

EFFECT OF EXTRACTING SOLVENT VOLUME ON RECOVERY RATE OF TWO VETERINARY ANTIBIOTICS ADDED TO SWINE MANURE

AUTHOR/S: E. PARENT, A. KARAM, L. E. PARENT
Monday 1 August 2011 by Libadmin2011

11th International Multidisciplinary Scientific GeoConference SGEM2011, www.sgem.org, SGEM2011 Conference Proceedings/ ISSN 1314-2704, June 20-25, 2011, Vol. 3, 283-288 pp

ABSTRACT

Land application of swine manure can increase antibiotic concentrations in agricultural
soils. It is thus important to develop laboratory methods for antibiotics in manures and
soils. The objective of this study was to examine the effect of volume of Na2EDTA–
McIlvaine’s buffer (citric acid and Na2HPO4) solution on recovery of oxytetracycline
(OTC) and chlortetracycline (CTC) added to fresh swine manure. Antibiotics were
mixed with manure at a single rate of 20 mg/L. Six treatments (8, 16, 24, 32, 40 or 48
ml) of extracting agents were used as 0.2 M Na2EDTA + 0.2 M citric acid + 0.4 M
Na2HPO4 solution to extract CTC and OTC from swine manure compost. Antibiotics
concentration in manure extracts was analyzed by high performance liquid
chromatography with ultraviolet detection. Compared with control (8 ml), the highest
rate (48 ml) of Na2EDTA-McIlvaine’s buffer solution increased the extraction recovery
of CTC and CTO by a factor of 1.8 and 1.4, respectively. There was lesser recovery of
CTC than OTC. At the levels of 8 and 48 ml of extracting solution mean recovery rates
were 59.7±14.2 and 81.8±16.0 % for OTC, and 36.1±12.1 and 65.7±0.1% for CTC,
respectively. The concentration of CTC and OTC in fresh swine manure compost must
account for a recovery factor that depended on volume of extracting solution.

Keywords: antibiotic extraction, McIlvaine’s buffer, chlortetracycline, oxytetracycline

PAPER 2011/s20.118: EFFECT OF EXTRACTING SOLVENT VOLUME ON RECOVERY RATE OF TWO VETERINARY ANTIBIOTICS ADDED TO SWINE MANURE

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