IMPROVEMENT OF METHODS OF BOVINE ANAPLASMOSIS DIAGNOSTICS

References: 13th SGEM GeoConference on Nano, Bio And Green – Technologies For A Sustainable Future, www.sgem.org, SGEM2013 Conference Proceedings, ISBN 978-619-7105-06-3 / ISSN 1314-2704, June 16-22, 2013, 163 - 168 pp

ABSTRACT

Specific antigens as well as RIDIF-active rabbit sera (1:320) used for detecting Ana-plasma in biomaterial of tick-transmitters and antibodies in serum of cattle, infected with anaplasmosis, have been received.
Obtained serum can be used as indicator and identificator of bovine anaplasmosis causa-tive agent in RIDIF and for studding antigenic and relative properties of bovine ana-plasmosis causative agent and disease pathogenesis.
High diagnostic value of RIDIF was confirmed practically, using this method (in com-bination with light microscopy) 14% infected animals as well as anaplasmosis – carriers have been detected.

Keywords: anaplasma, animals, specific antiserum and antigens, reaction of indirect immunofluorescence, Vero, D. reticulates, D. silvarum, hemolymph-test, biomaterial.