DBPapers
DOI: 10.5593/sgem2012/s14.v3011

THE DYNAMICS OF MARINE BACTERIONEUSTON IN LABORATORY MICROCOSMS: 2. THE INFLUENCE OF ORGANIC MATTER ADDITION

A. M. MOLDOVEANU, I. I. ARDELEAN
Wednesday 1 August 2012 by Libadmin2012

References: 12th International Multidisciplinary Scientific GeoConference, www.sgem.org, SGEM2012 Conference Proceedings/ ISSN 1314-2704, June 17-23, 2012, Vol. 3, 1009 - 1016 pp

ABSTRACT

The importance of the surface layer (bacterioneuston) for the accumulation of organic
compounds, surfactants and contaminants in the air- liquid interface are well-known.
The aim of this study was to quantify the dynamics of the bacterial cell density during
bacterioneuston formation at air-liquid interface in laboratory microcosms containing
sea water supplemented with concentrations of organic matter (0,1%, 0,5%, 1% peptone
and gasoline). The bacterial cells were analyzed using Acridine Orange for total cell
count under epifluorescence light. The results shown a bacterial density in the control
probe between 1.12 – 12.81 ·103 cells/mm2 , after organic additions in the experimental
seawater a higher bacterial cell density was obtain in the bacterioneuston formed at
0,1% peptone ( 3.60 – 25.65 ·103 cells/mm2 ), 0,5 % peptone ( 3.50 – 27.67 ·104
cells/mm2 ) and 1% peptone (values between 5.20 – 31.64 ·105 cells/mm2 ) than in the
seawater supplemented with 0,1% gasoline ( 2.3 – 14.86 ·103 cells/mm2 ), 0,5% gasoline
(2.3 - 21.6 ·103 cells/mm2) and 1% gasoline (with values between 2.51 - 23.63 ·104
cells/mm2).

Keywords: biofilm, interface, bacterioneuston, adherence, organic matter.