DOI: 10.5593/sgem2017H/63/S30.143


F. Cola, M. Cola
Thursday 23 November 2017 by Libadmin2017

References: 17th International Multidisciplinary Scientific GeoConference SGEM 2017, www.sgemviennagreen.org, SGEM2017 Vienna GREEN Conference Proceedings, ISBN 978-619-7408-29-4 / ISSN 1314-2704, 27 - 29 November, 2017, Vol. 17, Issue 63, 1153-1160 pp; DOI: 10.5593/sgem2017H/63/S30.143


Mastitis affects not only the health of milk-producing animals, having a direct consequence on the farm profitability, but it also affects the animals’ well-being. After performing the microbiologic examination of the milk samples coming from cows diagnosed with sub-clinical mastitis, were isolated 9 different species, out of which 7 species of bacteria and 2 species of fungi. The predominant species were those of staphylococci, Staphylococcus intermedius being isolated in 45,45% of the samples. The second species as frequency was Streptococcus agalactiae isolated in 31,81% of the samples, followed by Bacillus cereus with 27,27%. Mastitis usually has a negative influence on the milk-producing industry [2]. The spectrophotometric dosing of carotenoids is based on their capacity to absorb luminous radiation from the visible field, with an absorption maximum level placed around 450 450 nm. The quantitative analysis of retinol was made by means of a standard curve created with solutions of retinol-trans-total (Sigma) having the following concentrations: 5, 10, 14.28, 30, 50, 75, 100 μg/ml. The content of tocopherol in milk was accomplished at the same time with the retinol analysis. The monitoring of the chromatogram was accomplished at 295 nm and the quantitative determination was based on a standard curve created with solutions having concentrations of 5, 10, 14.28, 30, 50, 75, 100 μg/ml of α tocopherol. The identification of the antioxidant vitamins in the milk extracts was made by injecting in advance a mix of standards containing 100 mg/ml from every one of the three standards: α-retinol, δ-tocopherol and γ-tocopherol and by determining the retention time (tR) [1], [8].During our studies, we noticed the concentration decrease of the antioxidant vitamins both for retinol (13,87 μg/100 ml) and for tocopherols (50,27 μg/100 ml). By using the milk samples, we determined the content of total carotenoid and the content of B-carotene. If we make a comparison between normal milk (17,8 μg/100 ml and 19.3 μg/100 ml) and the milk coming from cows diagnosed with mastitis, we may notice that, in case of these conditions, the content of total carotenoids, respectively the content of β -carotene, is significantly lower, as it is placed under the detection limit.

Keywords: mastitis; cows; milk; antioxidant vitamins.